AFB Brilliant Green Counterstain is designed for use in acid-fast staining procedures, including the Kinyoun method, to provide strong contrast between acid-fast organisms and background material. When used after carbol fuchsin staining and decolorization, the counterstain produces a clean green background, allowing acid-fast bacteria to appear bright red for clear visualization. Suitable for routine use in clinical, veterinary, and research laboratories, this reagent is compatible with standard acid-fast staining protocols and manual procedures. The formulation supports consistent color development and reliable results in microbiology applications.